{"product_id":"biologix-western-transfer-buffer-10x-ph-8-3-500ml-bottle","title":"Biologix® Western Transfer Buffer ( 10X ), pH 8.3, 500mL\/ Bottle","description":"\u003cdiv style=\"font-family:Arial,Helvetica,sans-serif;color:#243746;line-height:1.65;max-width:1100px;margin:0 auto;\"\u003e\n\u003cdiv style=\"background:linear-gradient(135deg,#0067a3,#00a3d9);color:#fff;padding:26px 30px;border-radius:12px;margin:0 0 22px;\"\u003e\n\u003cp style=\"font-size:13px;font-weight:700;letter-spacing:1px;margin:0 0 8px;\"\u003eAUCKLAND SCIENTIFIC · BIOLOGIX\u003c\/p\u003e\n\u003ch2 style=\"color:#fff;font-size:26px;line-height:1.25;margin:0;\"\u003eBiologix® Western Transfer Buffer ( 10X ), pH 8.3, 500mL\/ Bottle\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size:16px;margin:0 0 20px;\"\u003eWestern transfer buffers are used in Western blotting experiments to transfer proteins from gels to membranes, and then to semi-quantitative detection of proteins in complex samples based on antigen-antibody specific binding. This product is a 10× concentrate and needs to be diluted as follows before use: Take 100 mL of 10× transfer buffer, add 200 mL of…\u003c\/p\u003e\n\u003cdiv style=\"border-left:4px solid #00a3d9;background:#f3f9fc;padding:16px 20px;margin:20px 0;\"\u003e\n\u003cstrong style=\"color:#0067a3;\"\u003eProduct information\u003c\/strong\u003e\u003cdiv style=\"margin-top:8px;\"\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eWestern transfer buffers are used in Western blotting experiments to transfer proteins from gels to membranes, and then to semi-quantitative detection of proteins in complex samples based on antigen-antibody specific binding.\n\t\u003c\/span\u003e\n\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 14px;\"\u003eThis product is a 10× concentrate and needs to be diluted as follows before use: Take 100 mL of 10× transfer buffer, add 200 mL of anhydrous methanol and mix well, then add distilled water or deionized water to make up to 1 L, mix well and it can be used.\u003c\/span\u003e\n\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\n\u003c\/p\u003e\n\u003cul\u003e\n\t\u003cli\u003eHigh quality: The buffers are filtered by 0.22 µm to ensure the highest quality.\n\t\u003c\/li\u003e\n\t\u003cli\u003eTime-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.\n\t\u003c\/li\u003e\n\t\u003cli\u003eDNase \u0026amp; RNase free, Protease-free, Pyrogen-free.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eComposition\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable class=\"ecwid-description-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\t\u003ctd\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003eTris\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\t\u003ctd style=\"text-align: center;\"\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003e250 mM\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\t\u003ctd style=\"text-align: center;\"\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003eGlycine\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\t\u003ctd style=\"text-align: center;\"\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003e1.92 M\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eProduct Properties\u003c\/strong\u003e\n\u003c\/p\u003e\n\u003ctable class=\"ecwid-description-table\"\u003e\n\u003cthead\u003e\n\u003c\/thead\u003e\n\u003c\/table\u003e\n\u003ctable class=\"ecwid-description-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\t\u003cth\u003eAppearance\n\t\u003c\/th\u003e\n\t\u003cth\u003epH\n\t\u003c\/th\u003e\n\t\u003cth\u003eSterile\n\t\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\t\u003ctd\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003eColorless clear liquid\u003cbr\u003e\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\t\u003ctd style=\"text-align: center;\"\u003e\n\t\t\u003cp style=\"text-align: center;\"\u003e8.3±0.1 (diluted to 1×)\u003cbr\u003e\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\t\u003ctd style=\"text-align: center;\"\u003eNon-sterile\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eStorage \u003c\/strong\u003e\u003cstrong\u003e\u0026amp;\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003eShelf\u003c\/strong\u003e\u003cstrong\u003e \u003c\/strong\u003e\u003cstrong\u003eLife\u003c\/strong\u003e\n\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003eStore at room temperature, valid for two years.\n\u003c\/p\u003e\n\u003cdiv\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eOrdering Information\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003ctable class=\"ecwid-description-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\t\u003ctd\u003e\n\u003cstrong\u003eCat #\u003c\/strong\u003e\n\t\u003c\/td\u003e\n\t\u003ctd\u003e\n\u003cstrong\u003eDescription\u003c\/strong\u003e\n\t\u003c\/td\u003e\n\t\u003ctd\u003e\n\u003cstrong\u003ePackage\u003c\/strong\u003e\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\t\u003ctd\u003eWTB-510-1\n\t\u003c\/td\u003e\n\t\u003ctd\u003e\n\t\t\u003cp\u003eWestern Transfer Buffer (10×)\n\t\t\u003c\/p\u003e\n\t\u003c\/td\u003e\n\t\u003ctd\u003e500mL\/ Bottle\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\t\u003ctd\u003eWTB-510\n\t\u003c\/td\u003e\n\t\u003ctd\u003eWestern Transfer Buffer (10×)\n\t\u003c\/td\u003e\n\t\u003ctd\u003e500mL\/ Bottle, 10 Bottles\/Case\n\t\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 style=\"color:#0067a3;border-bottom:2px solid #00a3d9;padding-bottom:7px;\"\u003eAvailable configurations\u003c\/h3\u003e\n\u003cp\u003eSelect from the priced configurations below. Each option is identified by its manufacturer SKU.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x:auto\"\u003e\u003ctable style=\"width:100%;border-collapse:collapse;border:1px solid #d4e2e8;\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#0077b6;color:#fff;\"\u003e\n\u003cth style=\"text-align:left;padding:11px;border:1px solid #d4e2e8;\"\u003eManufacturer SKU\u003c\/th\u003e\n\u003cth style=\"text-align:left;padding:11px;border:1px solid #d4e2e8;\"\u003eConfiguration\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\u003ctr\u003e\n\u003ctd\u003eWTB-510\u003c\/td\u003e\n\u003ctd\u003ePackage: 10 Bottles\u003c\/td\u003e\n\u003c\/tr\u003e\u003c\/tbody\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp style=\"font-size:12px;color:#68777d;margin-top:18px;\"\u003eProduct details supplied by Biologix. Product specifications may be subject to manufacturer updates.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Auckland Scientific","offers":[{"title":"10 Bottles","offer_id":59573732475205,"sku":"WTB-510","price":39.4,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1066\/1930\/6309\/files\/5086138086.jpg?v=1791121575","url":"https:\/\/auckland-scientific.co.uk\/products\/biologix-western-transfer-buffer-10x-ph-8-3-500ml-bottle","provider":"Auckland Scientific Ltd","version":"1.0","type":"link"}