{"product_id":"cmc1-horizontal-scale-10mm-0-1mm-25pcs-per-box","title":"Corrective Microscope Coverslips : CMC1 Horizontal Scale 10mm\/0.1mm (25pcs per box)","description":"\u003ch1 class=\"text-secondary\"\u003eCorrective Microscope Coverslips : \u003c\/h1\u003e\n\u003ch1 class=\"text-secondary\"\u003eCMC1 Horizontal Scale 10mm\/0.1mm\u003c\/h1\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"text-primary\"\u003e\u003cspan\u003ePattern: \u003c\/span\u003eCMC1\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"text-primary\"\u003e\u003cspan\u003eFilm Cover Slip with 10mm scale in 0.1mm (100µm) divisions.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"text-primary\"\u003e\u003cspan\u003eA unique film reticle for use in Correlative Microscopy. Designed specifically to allow identification and location of a particular area of interest under brightfield or fluorescence microscopes and then sectioning for electron microscopy.\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhysical and Chemical Characteristics\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e     Resistant to normal chemicals used in electron microscopy\u003cbr\u003e     No oxygen retention and compatible resin LR White\u003cbr\u003e     Good optical quality in brightfield \u0026amp; UV fluorescence\u003cbr\u003e     Excellent transparency\u003cbr\u003e     Does not deform at temperatures (positive 100°C and negative liquid N2)\u003cbr\u003e     Rigid, does not float in the middle of culture\u003cbr\u003e     Easy to handle and cut with a knife or micro-punch\u003cbr\u003e     Simple sterilisation using alcohol or UV\u003cbr\u003e     Detaches easily from resin after polymerisation\u003cbr\u003e     Low cost\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"text-primary\"\u003eDimensions: \u003c\/span\u003e22mm x 22mm\u003c\/p\u003e\n\u003cp\u003e\u003cspan class=\"text-primary\"\u003eMaterial and Thickness: \u003c\/span\u003ePolyester Film 0.18mm thick\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTechnical Advice\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eUse with Fluorescent Microscopes. These new correlative microscopy coverslips (CMC’s) are not designed to replace the glass or quartz coverslips, which have superior optical properties, and so are far better for fluorescent microscopy. The film itself has a structure which is visible under certain light conditions, especially with fluorescence. The CMC’s are designed to satisfy the needs of correlative microscopy where an initial, general analysis is performed using light or fluorescent microscopy and then the specimen is further processed for analysis by SEM, TEM or cryofixation.\u003c\/p\u003e\n\u003cp\u003eIf you need to perform detailed analysis using fluorescent microscopy then the CMC’s are not the solution. If however you want to perform a comprehensive analysis using different analysis techniques then the CMC’s are the only solution. The key advantage of the CMC’s is that they are made on special film that can be cut or punched (for cryo applications), something that is not possible with glass coverslips. The grid image is also transferred to any embedded specimen making cell location far easier.\u003c\/p\u003e\n\u003cp\u003eBest Types of Resin to Use. Because this is new technology it often requires a change in the preparation method. The method that technicians have traditionally used for glass coverslips may not be suitable for these film ones. The key thing to successful use of these CMC’s seems to be the type of resin and the preparation. We have already mentioned about the need to ensure a hermetic seal between the Beem capsule and the CMC film. The preparation works perfectly with low viscosity epoxy resins (Spurr, EPON). Sufficient polymerisation is obtained in 24 hours at 60 degrees Celsius – it does not normally require 48 hours.\u003c\/p\u003e\n\u003cp\u003eCleaning Procedure. Always clean the coverslips before applying the specimen. This can be done either with UV light or alcohol. After using alcohol it is best to immerse the coverslip in polysyline to give better adhesion.\u003cbr\u003e\u003c\/p\u003e","brand":"Auckland Scientific","offers":[{"title":"Default Title","offer_id":58326004695365,"sku":"01ACMC01","price":53.57,"currency_code":"GBP","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/1066\/1930\/6309\/files\/CMC1.jpg?v=1781956588","url":"https:\/\/auckland-scientific.co.uk\/products\/cmc1-horizontal-scale-10mm-0-1mm-25pcs-per-box","provider":"Auckland Scientific Ltd","version":"1.0","type":"link"}