Advanced LED Fluorescence Microscope

An advanced trinocular biological microscope designed for LED fluorescence and transmitted-light brightfield microscopy. It is available with either standard infinity-corrected plan achromatic objectives or high-aperture fluorescence objectives offering increased transmission, including at shorter wavelengths.

LED fluorescence illumination avoids the cost, warm-up time and handling considerations associated with conventional high-pressure mercury illumination. The system can be configured with one of four wavelength-specific fluorescence modules to suit the required fluorochromes.

Key features

• LED incident fluorescence microscopy
• Transmitted-light brightfield microscopy
• Trinocular viewing head with photo tube
• 50:50 visual and camera light split
• Widefield 10× eyepieces with FN22 field of view
• Infinity-corrected optical system
• Choice of plan achromatic or high-aperture fluorescence objectives
• Quintuple objective nosepiece
• Coarse and fine focusing controls
• Mechanical stage with ergonomically positioned controls
• Focusable and centreable condenser
• Condenser numerical aperture of 1.25
• Regulated 3.3 V, 3 W transmitted-light LED illumination
• Köhler illumination with field iris diaphragm
• Choice of four LED fluorescence wavelengths
• Fluorescence modules include excitation, dichroic and emission filters
• Suitable for microscope camera integration

Objective package options

Plan achromatic objective package

High-aperture fluorescence objective package

Optional 20×/0.50 and 100×/1.30 oil-immersion fluorescence objectives are available separately.

LED fluorescence wavelength options

Each fluorescence module includes the appropriate excitation filter, dichroic reflector and emission filter.

Fluorochrome compatibility should be confirmed against the precise excitation and emission characteristics of the intended stain or fluorescent marker.

Available configurations

Optional phase contrast

The microscope can also be configured for phase contrast observation. Available options include:

• 10× and 40× plan phase contrast objectives with matching phase slider
• 20× and 100× oil-immersion plan phase contrast objectives with matching phase slider
• Complete phase contrast outfit with 10×, 20×, 40× and 100× phase objectives
• Phase condenser
• Phase-centering telescope
• Green filter

I recommend describing phase contrast as an optional configuration available on request, rather than creating additional Shopify variants.

Other optional accessories

• Plan achromatic 50×/0.90 oil-immersion objective
• Plan achromatic 60×/0.85 dry objective
• Fluorescence 20×/0.50 objective
• Fluorescence 100×/1.30 oil-immersion objective
• Widefield focusing 10×/FN22 measuring eyepiece with scale graticule
• Calibrating stage micrometer with a 1 mm scale divided into 100 parts
• 0.3× and 0.5× C-mount camera adapters
• Compatible fluorescence and brightfield microscope cameras

Typical applications

• Fluorescence microscopy
• Immunofluorescence
• Cell and tissue imaging
• Microbiology
• Cytology and histology
• Fluorescent staining
• Live and fixed-cell research
• Biomedical research
• Pharmaceutical and life-science laboratories
• University teaching and research
• Digital fluorescence imaging

Please contact Auckland Scientific before ordering so that the fluorescence wavelength, objective package and camera configuration can be matched to your application.