CoolLED pE-100

Compact, precise and highly stable LED illumination for fluorescence microscopy, clinical screening, electrophysiology and research.

Select the wavelength required for your application

The CoolLED pE-100 is a compact single-wavelength LED illumination system available with a choice of excitation wavelengths from near-UV at 365 nm through to near-IR at 770 nm. It provides a controllable and reliable alternative to mercury, metal-halide and other conventional microscope light sources.

Dedicated Illumination for Specific Applications

The pE-100 is designed for applications requiring one carefully selected LED excitation wavelength. It is particularly well suited to routine clinical screening, fluorescence microscopy, electrophysiology, optogenetics and basic research.

Twenty LED wavelength options are available across the 365–770 nm range. The correct wavelength is selected according to the fluorophore, fluorescence filter set, fibre and intended application.

Operation is controlled through the remote control pod, providing instant on/off operation and precise irradiance adjustment in 1% steps. A TTL input enables rapid remote triggering for synchronisation with cameras and other experimental equipment.

20 Wavelength Options Select from wavelengths covering 365 nm to 770 nm.

Precise Control Irradiance adjustment in 1% steps from 0% to 100%.

TTL Triggering Remote triggering for synchronised fluorescence experiments.

Three Delivery Options Available in direct-fit, liquid-light-guide and fibre configurations.

Key Features

Dedicated single-wavelength LED illumination system
Choice of 20 wavelength options from 365 nm to 770 nm
Suitable for fluorescence microscopy, clinical screening and research
Precise irradiance control in 1% steps from 0% to 100%
Instant on/off operation with no warm-up or cool-down period
TTL triggering through a BNC connection on the control pod
Approximate TTL triggering speed of 150 microseconds
Direct-fit, liquid-light-guide and SMA fibre configurations
Stable and repeatable illumination for consistent results
Compatible with appropriate existing fluorescence filter sets
Excellent illumination uniformity across the field of view
Wide selection of microscope adaptors
Mercury-free and laser-free LED technology
No replacement bulbs or neutral-density filters required

Available Configurations

Configuration	Part Number	Description
Direct Fit	244-87-XXX-YYY-ZZ	Connects directly to the microscope epi-illumination port using a customer-specified fixed adaptor. Direct fitting provides high irradiance and requires a simple initial optical adjustment.
Liquid Light Guide	244-87-XXX-L15-ZZ	Supplied with a fixed 1.5 m long, 3 mm diameter liquid light guide for remote light delivery.
SMA Fibre	pE-100-F-XXX-ZZ	Provides an SMA connection for accepting a suitable multimode fibre. Fibre is not included.
Combined Dual Fibre System	pE-100-F-XXX-XXX-ZZ	Combines two pE-100 fibre light sources with independently selected wavelengths. Accepts 25 mm excitation filters. Fibre and filters are not included. COntact Auckland Scientific to confirm pricing.

Product-code configuration

XXX identifies the selected LED wavelength.
YYY identifies the customer-specified microscope adaptor.
ZZ identifies the regional power cable.

Auckland Scientific will confirm the complete part number after checking the required wavelength, microscope and light-delivery method.

Selecting the Correct Wavelength

The correct pE-100 wavelength depends on the fluorophore, excitation filter, dichroic mirror, application and required irradiance. Available wavelengths extend from near-UV at 365 nm through visible wavelengths to near-IR at 770 nm.

Wavelength selection should be confirmed before ordering. Auckland Scientific can review the application and fluorescence filter specifications to identify the most appropriate LED option.

What Is Included?

CoolLED pE-100 LED light source at the selected wavelength
Remote manual control pod
Power supply
UK power cable
Customer-specified fixed microscope adaptor with direct-fit systems
Fixed 1.5 m, 3 mm liquid light guide with liquid-light-guide systems
SMA fibre connection with fibre configurations

A microscope, fluorescence filter set, fibre and other imaging equipment are not included unless expressly stated.

Optional Accessories & Combined Systems

Accessory	Part Number	Price
pE-Combiner for two pE-100 light sources, including a CoolLED-selected dichroic mirror, two excitation filter holders and adaptor for the specified microscope	244-93-XXX-XXX-YYY	Price on Request
pE-Combiner for two pE-100 light sources, including a CoolLED-selected dichroic mirror and adaptor for the specified microscope	244-94-XXX-XXX-YYY	Price on Request
pE-Universal Collimator with customer-specified microscope adaptor	pE-10400-YYY	Price on Request

Control Options

Manual control	Instant on/off and irradiance adjustment in 1% steps from 0% to 100%
Remote control	Single TTL input through a BNC connection on the control pod
Triggering speed	Approximately 150 microseconds

Technical Specifications

Wavelength range	Choice of 20 LED wavelengths from 365 nm to 770 nm
Irradiance control	0% to 100% in 1% increments
Power requirement	100–240 V AC, 50/60 Hz
Standby consumption	2.2 W
Full irradiance consumption	25 W
Direct-fit light source	60 × 170 × 70 mm; approximately 0.38 kg
Light-guide light source	52 × 95 × 52 mm; approximately 0.42 kg
Fibre light source	76 × 165 × 80 mm; approximately 0.42 kg
Standard warranty	Three-year CoolLED manufacturer’s warranty

Typical Applications

Routine single-channel fluorescence microscopy
Clinical fluorescence screening
Auramine-based tuberculosis screening
Fungal and bacterial screening
Electrophysiology
Optogenetics
Calcium-indicator imaging
Basic fluorescence research
Fibre-coupled illumination
Replacement of mercury and metal-halide illumination

Brochures & Technical Resources

View the CoolLED pE-100 brochure and supporting product information in the Auckland Scientific Technical Resource Centre.

View Brochure & Technical Resources

Experienced Microscopy Support

Auckland Scientific brings over 21 years of microscopy sales and application experience. We provide knowledgeable, practical support to help customers select the correct LED wavelength, fluorescence filter compatibility, light-delivery method and microscope adaptor. Support is available from initial product selection through to installation, training and ongoing service.

Configuration Support

Please provide the application, fluorophore, fluorescence filter details, microscope manufacturer and model when requesting a quotation. Auckland Scientific will help confirm the appropriate LED wavelength, light-delivery configuration and adaptor.

Microscope, fluorescence filter sets, fibres and imaging equipment are not included unless expressly stated. Product appearance and specifications may be updated by the manufacturer without notice. Configuration must be confirmed before ordering.