CoolLED pE-300lite LED Fluorescence Microscope Illumination System
Mercury-Free Fluorescence Illumination
CoolLED pE-300lite
Simple, powerful broad-spectrum LED illumination for routine fluorescence microscopy.
The CoolLED pE-300lite provides intense, stable and repeatable LED illumination across the excitation bands of commonly used fluorophores, from DAPI through to Cy5. Instant operation eliminates warm-up and cool-down periods, while precise irradiance control helps protect sensitive samples.
Designed for Everyday Fluorescence Microscopy
The pE-300lite is an accessible white-light fluorescence illumination system designed for hospital laboratories, clinical screening, teaching facilities and routine research microscopy.
Its broad spectral output supports popular fluorophores including DAPI, CFP, Aqua, FITC, YFP, TRITC, Texas Red, mCherry and Cy5. The remote control pod provides instant on/off operation and fine irradiance adjustment without mechanical shutters or neutral-density filters.
Key Features
- Intense broad-spectrum LED illumination for fluorescence microscopy
- Covers commonly used fluorophores from DAPI to Cy5
- Stable and repeatable illumination for consistent results
- Instant on/off operation with no warm-up or cool-down period
- Fine irradiance control in 1% steps from 0% to 100%
- Excellent illumination uniformity across the field of view
- No replacement bulbs or routine lamp alignment
- Compatible with single-band and multi-band fluorescence filter sets
- Direct-fit and liquid-light-guide configurations available
- Wide selection of adaptors for current and older microscopes
- Low power consumption and long LED operating lifetime
- Mercury-free and laser-free illumination technology
Select the Correct Configuration
| Configuration | Recommended When |
|---|---|
| Direct Fit | The illuminator can connect directly to the microscope. Direct fitting provides higher irradiance and avoids the ongoing replacement cost associated with liquid light guides. |
| Liquid Light Guide | The illumination source needs to be positioned remotely from the microscope, or the system is replacing an existing light-guide-coupled lamp. |
| Single-Band – SB | The microscope uses single-band filter sets, typically with DAPI excitation at approximately 365 nm. |
| Multi-Band – MB | The microscope uses a multi-band fluorescence filter set, typically with DAPI excitation at approximately 400 nm. |
The correct light-delivery method, SB or MB configuration and microscope adaptor must be confirmed before ordering. Auckland Scientific can help identify the correct system for your microscope and installed fluorescence filter sets.
What Is Included?
- CoolLED pE-300lite LED light source
- Remote manual control pod
- Power supply
- UK power cable
- Light-source stand with light-guide configurations
- Customer-specified microscope adaptor with direct-fit configurations
A liquid light guide, universal collimator and microscope adaptor may be required for light-guide configurations. These components are selected separately according to the microscope and installation requirements.
Technical Specifications
| Illumination type | Broad-spectrum LED fluorescence illumination |
|---|---|
| Spectral coverage | UV DAPI excitation through to the red Cy5 region |
| Irradiance control | 0% to 100% in 1% increments |
| Control | Remote manual control pod |
| Light delivery | Direct fit or 3 mm liquid light guide |
| Power requirement | 100–240 V AC, 50/60 Hz |
| Standby power consumption | 3.3 W |
| Full white irradiance consumption | 56.4 W at the wall, including power supply |
| Light-source dimensions | 77 × 186 × 162 mm |
| Light-source weight | Approximately 1.4 kg |
| Control-pod dimensions | 88 × 125 × 37 mm |
| Warranty | Three-year CoolLED manufacturer’s warranty |
Typical Applications
- Routine fluorescence microscopy
- Clinical and hospital laboratory screening
- Pathology and diagnostic microscopy
- University teaching and research laboratories
- Widefield fluorescence imaging
- Pre-scan sample viewing on confocal microscopes
- Replacement of mercury and metal-halide illuminators
Brochures & Technical Resources
View the CoolLED pE-300 Series brochure and supporting product information in the Auckland Scientific Technical Resource Centre.
View Brochure & Technical ResourcesAuckland Scientific Configuration Support
Please provide the microscope manufacturer, model and details of the installed fluorescence filter sets when requesting a quotation. We will help confirm the correct adaptor, light-delivery method and SB or MB configuration.
Microscope, fluorescence filter sets and imaging equipment are not included. Product appearance and specifications may be updated by the manufacturer without notice. Extended warranty options are available when ordered with the system.