CoolLED pE-340fura LED Illumination System for Fura-2 Calcium Imaging

Direct Fit

£9,795.00

CoolLED pE-340fura LED Illumination System for Fura-2 Calcium Imaging

£9,795.00

High-Speed Ratiometric Calcium Imaging

CoolLED pE-340^fura

Dedicated 340 nm and 380 nm LED illumination for high-precision Fura-2 calcium imaging, with broad white output for routine fluorescence microscopy.

Purpose-built for Fura-2 ratiometric imaging

The CoolLED pE-340^fura combines dedicated 340 nm and 380 nm excitation with fast channel switching, stable LED output and independent irradiance control. It is designed for Fura-2 ratiometric calcium imaging while also providing broad white output for everyday fluorescence applications.

Fast, Stable Calcium Imaging

Fura-2 calcium imaging requires accurate and repeatable switching between 340 nm and 380 nm excitation. The pE-340^fura replaces slower mechanical wavelength switching with independently controlled LEDs capable of triggering in less than 20 microseconds.

Stable LED illumination helps reduce experimental noise and improves the precision of ratiometric measurements. Increased signal-to-noise performance may also allow lower concentrations of Fura-2 dye to be used, helping reduce dye toxicity and improve cell viability.

In addition to calcium imaging, the system provides broad white output from approximately 435–645 nm for imaging standard fluorophores including GFP, FITC, TRITC, Texas Red, mCherry and Cy5.

340 & 380 nm Dedicated excitation wavelengths for Fura-2 ratiometric imaging.

Microsecond Triggering Global and individual channel switching in less than 20 microseconds.

Broad White Output Supports routine fluorescence imaging from approximately 435–645 nm.

Sequence Runner Run a user-defined excitation sequence from a single TTL output.

Key Features

Dedicated 340 nm and 380 nm excitation for Fura-2 imaging
Broad white output from approximately 435–645 nm
Global and individual channel TTL triggering
Triggering speed of less than 20 microseconds at full power
Sequence Runner for user-defined excitation sequences
Independent channel selection and irradiance control
Control in 1% steps from 0% to 100%
Stable LED output for precise ratiometric measurements
High signal-to-noise performance
UV-optimised optics for efficient 340 nm transmission
Three removable 25 mm excitation filter holders
340 nm and 380 nm excitation filters supplied
Direct-fit and UV liquid-light-guide configurations available
USB connectivity for compatible imaging-software integration
Mercury-free, laser-free and energy-efficient operation

Sequence Runner

Sequence Runner allows a user-defined sequence of excitation channels to be initiated through a single TTL output from a camera or other external device. This provides rapid synchronised imaging without requiring multiple TTL outputs or costly additional control hardware.

Available Configurations

Configuration	Part Number	Description
Direct Fit	pE-340-FR-D-YYY-ZZ	Connects directly to the microscope through a customer-specified adaptor. Direct fitting provides high irradiance and efficient UV transmission.
UV Liquid Light Guide	pE-340-FR-L-ZZ	Designed for use with a 3 mm UV-compatible liquid light guide. A UV-optimised collimator and microscope adaptor may also be required.

UV-compatible light delivery is essential

Standard liquid light guides and optical components may not transmit 340 nm efficiently. Auckland Scientific will confirm the correct UV-compatible light guide, collimator and microscope adaptor for the intended system.

What Is Included?

CoolLED pE-340^fura LED light source
Remote manual control pod
Three removable excitation filter holders for 25 mm filters
Excitation filters for 340 nm and 380 nm
Power supply
UK power cable
Customer-specified microscope adaptor with direct-fit systems
Light-source stand with liquid-light-guide systems

The liquid light guide and UV-optimised microscope collimator are selected separately for light-guide configurations.

Optional Accessories

Accessory	Part Number	Price
1.5 m long, 3 mm diameter liquid light guide	pE-1906	Price on Request
3 m long, 3 mm diameter liquid light guide	pE-1908	Price on Request
pE-340fura UV Universal Collimator with customer-specified microscope adaptor	pE-340-FR-COLL-YYY	Price on Request
USB-TTL conversion kit	pE-6501	Price on Request
USB-TTL conversion kit when ordered with the illumination system	pE-6501S	Price on Request

YYY denotes the customer-specified microscope adaptor. UV compatibility must be confirmed before ordering a light guide.

Warranty & Extended Coverage

The pE-340^fura is supplied with a three-year CoolLED manufacturer’s warranty. The 340 nm LED is warranted for 3,000 accumulated operating hours.

Warranty Option	Part Number	Price
Extension to four years of total warranty coverage	pE-340-FR-EXT-WRN-1	Price on Request
Extension to five years of total warranty coverage	pE-340-FR-EXT-WRN-2	Price on Request

Extended warranty coverage must be ordered at the same time as the illumination system.

Control Options

Manual control	Independent channel selection and irradiance control in 1% steps
TTL triggering	Global and individual channel inputs through BNC connections
Triggering speed	Less than 20 microseconds at full power
Software connectivity	USB Type-B connection for compatible imaging software
Sequence Runner	User-defined channel sequence initiated through a single TTL input

Technical Specifications

Excitation channels	340 nm, 380 nm and broad white output
White spectral coverage	Approximately 435–645 nm
Irradiance control	0% to 100% in 1% increments
Power requirement	100–240 V AC, 50/60 Hz
Maximum consumption	36 W with all three bands at full irradiance
Light-source dimensions	77 × 186 × 162 mm; approximately 1.40 kg
Control-pod dimensions	88 × 125 × 37 mm; approximately 0.32 kg
Standard warranty	Three years; 340 nm LED limited to 3,000 accumulated operating hours

Typical Applications

Fura-2 ratiometric calcium imaging
Live-cell calcium measurements
High-speed cellular signalling studies
Electrophysiology and patch-clamp recording
Cardiac and neuronal calcium imaging
Video-rate fluorescence acquisition
Routine multi-colour fluorescence microscopy
Quantitative fluorescence experiments

Brochures & Technical Resources

View the CoolLED pE-340fura brochure and supporting product information in the Auckland Scientific Technical Resource Centre.

View Brochure & Technical Resources

Experienced Microscopy Support

Auckland Scientific brings over 21 years of microscopy sales and application experience. We provide knowledgeable support to help customers configure the correct illumination system, UV-compatible light delivery, microscope adaptor and imaging-software integration. Support is available from initial specification through to installation, commissioning, training and ongoing service.

Configuration & Integration Support

Please provide the microscope model, camera, imaging software, fluorescence filters and triggering requirements when requesting a quotation. Auckland Scientific will help confirm the correct light-delivery method, adaptor and control configuration.

Microscope, camera, liquid light guide and imaging equipment are not included unless expressly stated. Product appearance and specifications may be updated by the manufacturer without notice. Extended warranty options must be ordered with the illumination system.