Prior Scientific PureFocus 850 Laser Autofocus System
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Prior Scientific PureFocus 850 Laser Autofocus System
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Prior Scientific Hardware Autofocus
PureFocus 850 Laser Autofocus System
Rapid, real-time hardware autofocus for compatible industrial, materials-science and life-science microscopy systems.
Price on quotation
PureFocus 850 must be configured for the microscope, optical system, objectives, sample interface, focus actuator, operating mode and software requirements. Auckland Scientific will review the complete application and provide a system-specific quotation.
Product overview
Prior Scientific PureFocus 850 combines advanced optics and intelligent processing to provide rapid, real-time focus maintenance for compatible infinity-corrected microscope systems.
The system detects a reflective reference interface within the sample arrangement and continuously maintains the required distance between that reference and the imaging focal plane. This helps compensate for focus drift caused by temperature changes, mechanical movement, sample variation or long-duration image acquisition.
A motorised offset lens allows the imaging depth to be adjusted relative to the detected interface. This enables the system to maintain a defined imaging plane or acquire images at different focus offsets while continuing to reference the same surface.
PureFocus 850 is installed within the infinity space of a compatible optical system. It can be integrated with selected commercial microscopes, Prior OpenStand systems, Queensgate piezo devices and supported third-party focus actuators.
Real-time autofocus
Rapidly detects and maintains focus during automated imaging and long-duration acquisitions.
Long recapture range
Provides a long objective-dependent capture range for recovering focus after larger movements.
Dynamic laser adjustment
Dynamic power adjustment supports reliable detection across different samples and reflective interfaces.
Multi-objective operation
Stores and recalls objective-specific parameters for systems using multiple objectives.
See PureFocus 850 in operation
Watch Prior Scientific’s introduction to the PureFocus 850 laser autofocus system and see how it provides rapid, real-time focus control for automated microscopy.
How PureFocus works
- The 850 nm laser is directed through the microscope’s infinity optical path towards the sample.
- PureFocus detects a suitable reflective interface, such as a slide surface, coverslip, glass-bottom dish or reflective material surface.
- The system calculates the focus correction needed to maintain the selected imaging plane.
- A compatible motorised focus actuator responds to the correction signal and adjusts the microscope focus.
- Focus is continuously monitored and corrected during imaging.
Available PureFocus 850 configurations
PureFocus 850 is available with different surface-tracking modes and controller arrangements. These options are selected during system configuration and are not Shopify variants.
| Part number | Tracking mode | Included controller | Typical use |
|---|---|---|---|
| PF850 | Slice mode | PF100 human-interface controller | Focus detection from approximately the centre of the field of view |
| PF850M | Line mode | PF100 human-interface controller | Focus determined from an averaged signal across part of the field of view |
| PF850OEM | Slice mode | PF301 compact OEM controller | OEM or software-controlled instrument integration |
| PF850MOEM | Line mode | PF301 compact OEM controller | OEM line-mode autofocus integration |
Slice mode
Determines the focus position from a point around the centre of the field of view. It may be suitable where a defined central reference area is required.
Line mode
Determines focus from an averaged detected signal across a proportion of the field of view. It may provide more representative surface tracking for certain samples.
Controller options
| Controller | Interface | Focus control | Application |
|---|---|---|---|
| PF100 | Human-interface controller with adjustment control and USB-B | One stepper or piezo Z-axis port | Operator-accessible standalone and integrated systems |
| PF301 | Compact black-box OEM controller with USB-B | One stepper or piezo Z-axis port | Software-controlled and OEM integration |
A separate focus actuator is required
PureFocus detects and calculates the required focus correction, but a compatible motorised microscope focus drive, piezo focus device or other supported actuator is required to physically move the focal plane. The focus actuator is not included unless specifically stated in the quotation.
Principal specifications
| Laser wavelength | 850 nm |
|---|---|
| Laser classification | Class 1 laser device |
| Laser optical output | Less than 0.77 mW |
| Dichroic clear aperture | 32 mm diameter |
| Focus accuracy | 20% of depth of field |
| Typical update rate | 1 kHz |
| Transmitted wavelength range | 365–800 nm |
| Transmission | Greater than 85% across the specified transmitted range |
| Laser module mass | 1.5 kg |
Typical capture performance
| Objective example | Typical capture speed | Capture range |
|---|---|---|
| 5×, NA 0.13 | Less than 30 ms | ±15,000 µm |
| 10×, NA 0.4 | Less than 50 ms | ±3,000 µm |
| 20×, NA 0.5 | Less than 60 ms | ±1,000 µm |
| 40×, NA 0.95 | Less than 100 ms | ±500 µm |
| 100×, NA 0.9 | Less than 1,500 ms | ±100 µm |
Capture speed and range figures are Prior’s published examples. Actual performance depends on the objective, numerical aperture, sample, reflective interface, optical configuration and focus actuator.
Published microscope compatibility
Prior publishes the following microscope and adapter combinations. The exact microscope configuration must be checked before ordering.
| Manufacturer | Compatible microscopes | Adapter |
|---|---|---|
| Evident/Olympus | BX53M, MX50, MX51, MX61, MX61L, MX63 and MX63L | LF335 |
| Evident/Olympus | IX73 two-deck configuration | PF208 |
| Evident/Olympus | IX73 one-deck configuration | PF214 |
| Evident/Olympus | IX71 | PF203 |
| Evident/Olympus | IX81 | PF213 |
| Evident/Olympus | BX51 and BX53 | PF210 |
| Nikon | LV100ND, LV100NDA, LV150N, LV150NA, L200N, L200ND, L300N, L300ND and Ni-U | LF320 |
| Nikon | Ti-U | PF211 |
| Nikon | Ti2-U and Ti2-A | PF212 |
| Leica | DM4000, DM5000, DM6000 and DM6 | LF312 |
| Zeiss | Axioskop 40 | LF341 |
| Prior Scientific | OpenStand-U, OpenStand-M, OpenStand-L and OpenStand-V | OpenStand-specific PureFocus adapter to be confirmed |
Additional compatibility checks: contact Auckland Scientific regarding Nikon Ti2-E, Zeiss Axio Imager models, an IX73 with both decks occupied, or any microscope not shown above.
Typical applications
- Automated slide scanning
- Long-duration time-lapse microscopy
- Live-cell imaging using suitable glass-bottom vessels
- Multiwell-plate imaging
- Semiconductor and wafer inspection
- Metallurgical and materials analysis
- Geological sample imaging
- Automated industrial inspection
- OEM imaging instruments
Software and system integration
PureFocus 850 can operate as part of a standalone microscope system or be integrated into a wider automated imaging platform.
Prior’s compatibility information identifies PureFocus support within µManager and Inscoper. Software compatibility depends on the controller, connected focus actuator, operating system, software version and required functions.
The PF100 and PF301 controllers provide USB connectivity for computer integration. If Python or other custom control is required, Auckland Scientific will confirm the applicable Prior interface, documentation and software-development support before quotation.
Setup tools and accessories
| Part number | Description |
|---|---|
| PF209 | PureFocus setup sample slide |
| PF300 | Setup camera with visible and near-infrared detection |
| PF200 | Setup target for RMS, W26 and M32 objective threads |
| PF201 | Setup target for M25, M27 and M32 objective threads |
| PF400 | 1.5 m PureFocus module-to-controller cable |
| PF404 | 1.5 m focus-control cable with 15-way D-type connection |
Product literature and downloads
Download the available PureFocus 850 technical information below.
Supported by Auckland Scientific
Auckland Scientific brings more than 21 years of experience in microscopy sales, imaging applications and scientific instrumentation. PureFocus is an application-dependent system, so we review the complete optical and automation arrangement rather than specifying the autofocus module in isolation.
Our service can include:
- Application and sample-interface assessment
- Microscope and optical-path compatibility checks
- Slice-mode or line-mode selection
- Focus-actuator and controller configuration
- Objective and wavelength review
- Installation and commissioning
- System setup and operator training
- Software and workflow guidance
- Ongoing technical and service support
Request a quotation
To help us assess and configure your PureFocus system, please provide:
- The microscope manufacturer, model and optical configuration
- Whether the microscope is upright or inverted
- The objectives and numerical apertures being used
- The sample type and reflective reference interface
- The illumination and imaging wavelengths
- The existing motorised focus or piezo actuator
- Whether human-interface or OEM control is required
- The imaging or automation software
- Any installation, training or custom-integration requirements
Auckland Scientific will review the application and provide a quotation covering the PureFocus module, controller, microscope adapter, focus actuator, setup components, cables and support services required.
PureFocus performance depends on the microscope, objectives, optical configuration, sample interface and focus actuator. Specifications and compatibility information may change. Final suitability and the complete system configuration will be confirmed at quotation stage.