AUC-291 Series Trinocular Inverted Biological LED Microscopes with illumination Options
Auckland Scientific
AUC-291 Series Trinocular Inverted Biological Microscopes
Advanced long-working-distance microscopy for cultured cells, tissues and live-cell observation
The AUC-291 Series combines an infinity colour-corrected optical system, ergonomic trinocular viewing and long-working-distance optics for the observation of living cells, microorganisms and tissue cultures. Choose the AUC-291-I for transmitted-light brightfield and positive phase contrast, or the AUC-291F-I for transmitted-light observation plus reflected LED fluorescence.
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Choose your configuration
AUC-291-I
Transmitted brightfield and positive phase contrast
- 4×, 10×, 20× and 40× infinity LWD plan achromatic positive phase-contrast objectives
- Transmitted biological frame with adjustable 5W LED illumination
- Fixed stage with Petri-dish holder, glass plate and Φ25mm metal plate
- Green filter and centring telescope
AUC-291F-I
Transmitted light, positive phase contrast and reflected LED fluorescence
- 4× and 10× infinity LWD plan fluorescence objectives
- 20× and 40× infinity LWD plan semi-apochromatic positive phase-contrast fluorescence objectives
- Four-channel LED fluorescence illuminator with B1 and G1 modules
- Attachable mechanical stage, extension stage and expanded specimen-holder set
Key features
- Infinity colour-corrected optical system for clear, flat and well-corrected images.
- PL10×/22mm high-eyepoint eyepieces provide a wide, comfortable field of view.
- Ergonomic trinocular head, inclined 45°, rotatable through 360° and adjustable for 50–76mm interpupillary distance.
- Inward-facing quintuple nosepiece accommodates the four supplied objectives and leaves one position available.
- 72mm condenser working distance supports observation through culture vessels.
- Intelligent ECO illumination uses an infrared sensor to control the light when the microscope is unattended.
- Coaxial coarse and fine focus with 0.002mm fine division.
- Trinocular imaging capability; compatible C-mount adapters and cameras are available separately.
Model comparison
| Feature | AUC-291-I | AUC-291F-I |
|---|---|---|
| Observation methods | Brightfield and positive phase contrast | Brightfield, positive phase contrast and LED fluorescence |
| Viewing split | Eyepiece : trinocular = 80:20 | Eyepiece : trinocular = 0:100 or 100:0 |
| Objectives supplied | 4×, 10×, 20×, 40× LWD plan achromatic positive phase contrast | 4×, 10× LWD plan fluorescence; 20×, 40× LWD semi-apochromatic positive phase-contrast fluorescence |
| Illumination | Adjustable 5W transmitted LED | Independently controlled transmitted and reflected LED |
| Stage equipment | Fixed stage, Φ35mm Petri-dish holder, glass plate, Φ25mm metal plate | Fixed stage, attachable mechanical stage, extension stage, Φ35mm Petri-dish holder, glass-slide holder, Terasaki holder, glass plate and kidney-shaped metal plate |
| Fluorescence modules | Not applicable | B1 blue excitation and G1 green excitation |
| C-mount adapter | Optional | Optional |
Objective specifications
| Model | Objective | NA | Working distance |
|---|---|---|---|
| AUC-291-I | 4× positive phase contrast | 0.13 | 10.75mm |
| 10× positive phase contrast | 0.25 | 7.45mm | |
| 20× positive phase contrast | 0.40 | 6.92mm | |
| 40× positive phase contrast | 0.65 | 2.74mm | |
| AUC-291F-I | 4× plan fluorescence | 0.13 | 18.95mm |
| 10× plan fluorescence | 0.30 | 7.27mm | |
| 20× semi-apochromatic positive phase contrast fluorescence | 0.45 | 6.12mm | |
| 40× semi-apochromatic positive phase contrast fluorescence | 0.65 | 1.79mm |
AUC-291F-I fluorescence system
The four-channel LED fluorescence illuminator includes 385nm, 470nm and 560nm LED light sources and is supplied with the following two filter modules:
- B1 blue-excitation module: EX 480/30 (Φ25mm), BS 505 (25.7 × 36 × 1mm), EM 535/40 (Φ25mm), for a 470nm centre-wavelength LED.
- G1 green-excitation module: EX 560/40 (Φ25mm), BS 595 (25.7 × 36 × 1mm), EM 635/60, for a 560nm centre-wavelength LED.
An eye-protection plate is included. The system offers long LED life, low heat output and avoids the warm-up and lamp-replacement demands associated with traditional fluorescence illumination.
Shared technical specification
| Optical system | Infinity colour-corrected optical system |
|---|---|
| Eyepieces | PL10×/22mm high-eyepoint wide-field plan eyepieces with diopter adjustment |
| Nosepiece | Inward-facing quintuple nosepiece |
| Condenser | Detachable NA 0.3 LWD condenser, 72mm working distance, with phase-adjustable sliding plate |
| Stage | Fixed stage, 215 × 250mm; model-specific holders as detailed above |
| Focusing | Coaxial coarse and fine focus with tension adjustment; 0.002mm fine division; 9mm movement range |
| Power input | 100–240V external wide-voltage power supply |
Applications
Suitable for live-cell and tissue-culture observation in universities, research institutes, medical and healthcare laboratories. Typical fields include cytology, microbiology, parasitology, oncology, immunology, genetic engineering, industrial microbiology and botany.
Three-year Auckland Scientific microscope warranty
Both variants are supplied with a three-year Auckland Scientific microscope warranty, subject to the microscope receiving an annual service. Full warranty terms are available on request.
Select your model and order online
Choose AUC-291-I or AUC-291F-I from the product options above. Prices are displayed separately for each variant. Delivery, installation and training are not included in the product price and will be quoted separately where required.
Telephone: 07853 690001
Email: michael.hallewell@auckland-scientific.co.uk
Website: www.auckland-scientific.co.uk