Biologix® Tris-glycine-SDS Buffer ( 1X ), pH8.3, 500mL/Bottle,10 Bottles/Case
£39.40
Sale price
£39.40
Regular price
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Biologix® Tris-glycine-SDS Buffer ( 1X ), pH8.3, 500mL/Bottle,10 Bottles/Case
£39.40
Sale price
£39.40
Regular price
AUCKLAND SCIENTIFIC · BIOLOGIX
Biologix® Tris-glycine-SDS Buffer ( 1X ), pH8.3, 500mL/Bottle,10 Bottles/Case
Tris-glycine-SDS (TGS) electrophoresis buffer contains SDS and is commonly used as a buffer for denatured protein polyacrylamide gel electrophoresis. Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) is the electrophoresis of denatured proteins on polyacrylamide gels with the addition of denaturants such as SDS and ethanol. SDS is an anionic…
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Tris-glycine-SDS (TGS) electrophoresis buffer contains SDS and is commonly used as a buffer for denatured protein polyacrylamide gel electrophoresis. Denaturing polyacrylamide gel electrophoresis (SDS-PAGE) is the electrophoresis of denatured proteins on polyacrylamide gels with the addition of denaturants such as SDS and ethanol.
SDS is an anionic surfactant. As a denaturant and solubilizing agent, it can break the hydrogen bonds within and between molecules, unfold the molecules, and destroy the secondary and tertiary structures of protein molecules. Strong reducing agents such as mercaptoethanol and dithiothreitol can break the disulfide bonds between cysteine residues. After adding reducing agents and SDS to the sample and gel, the molecules are depolymerized into polypeptide chains. The depolymerized amino acid side chains and SDS combine to form protein-SDS micelles. The negative charge carried by them greatly exceeds the original charge of the protein, thus eliminating the charge differences and structural differences between different molecules. The mobility of the protein mainly depends on its relative molecular mass.
Features
High quality: The buffers are filtered by 0.22 µm to ensure the highest quality.
Time-saving and error-avoiding: Pre-made electrophoresis buffers shorten preparation time while reducing the likelihood of buffer concentration errors.
DNase & RNase free, Protease-free, Pyrogen-free.
Composition
|
Tris |
25 mM |
|
Glycine |
192 mM |
|
SDS |
0.1%（W/V） |
Product Properties
|
Appearance |
Colorless clear liquid |
|
pH |
8.3±0.1 |
Storage & Shelf Life
Store at room temperature, valid for 2 years.
| Cat # | Description | Package |
| TGS-500 |
TG ( Tris-glycine ) Electrophoresis Buffer (1×) |
500mL/ Bottle,10 Bottles/Case |
Available configurations
Select from the priced configurations below. Each option is identified by its manufacturer SKU.
| Manufacturer SKU | Configuration |
|---|---|
| TGS-500 | Standard configuration |
Product details supplied by Biologix. Product specifications may be subject to manufacturer updates.