CoolLED pE-400max Four-Channel High-Speed LED Fluorescence Illumination System
Individually Controlled Four-Channel Illumination
CoolLED pE-400max
Fast, flexible LED illumination with individual channel control, microsecond triggering and Sequence Runner automation.
The CoolLED pE-400max combines four powerful, independently controlled LED channels with broad spectral coverage from approximately 365–635 nm. It supports popular fluorophores from DAPI through YFP to Cy5 and provides the speed and control required for demanding fluorescence, live-cell and optogenetics applications.
High-Speed Multi-Channel Illumination
Each of the four LED channels can be selected, triggered and adjusted independently, with any combination of channels operating simultaneously. Global and individual TTL inputs provide switching in less than 10 microseconds.
Optional inline excitation filter holders allow single-band excitation filters to be placed within the light source. When combined with an appropriate multi-band filter cube, this can remove the need for a mechanical filter wheel and its associated switching delays, vibration and cost.
Control is available through the manual control pod, compatible third-party imaging software and the CoolLED LightBridge graphical interface.
Key Features
- Four independently controllable LED channels
- Any combination of channels can operate simultaneously
- Broad spectral coverage from approximately 365–635 nm
- Supports popular fluorophores from DAPI through YFP to Cy5
- Global and individual channel TTL triggering
- Triggering speed of less than 10 microseconds at full power
- Sequence Runner for rapid user-defined excitation sequences
- Individual channel irradiance control in 1% steps
- Optional inline excitation filter holders
- Potential to replace mechanical filter-wheel configurations
- Manual control pod for straightforward local operation
- USB Type-B connectivity for compatible imaging software
- LightBridge graphical user interface
- Direct-fit and liquid-light-guide delivery options
- Single-band and multi-band DAPI configurations
- In-field firmware upgrade capability
- Mercury-free, laser-free and energy-efficient operation
Sequence Runner Automation
Once an LED and irradiance sequence has been defined, Sequence Runner automatically cycles through the selected channels. Synchronisation is initiated through the global TTL input using a single TTL output from a camera or other device, providing high-speed automation without complex additional control hardware.
LightBridge Graphical Control
The CoolLED LightBridge graphical interface provides an additional level of control for the pE-400max, including:
- LED on/off control
- Individual LED selection
- Real-time irradiance adjustment
- Sequence Runner configuration
- Save and load experimental presets
- Configure system start-up settings
SB or MB Filter Configuration
| Configuration | UV LED | Typical Use |
|---|---|---|
| Single-Band – SB | 365 nm | Microscopes using single-band fluorescence filters with DAPI excitation around 365 nm. |
| Multi-Band – MB | 400 nm | Microscopes using multi-band fluorescence filters with DAPI excitation around 400 nm. |
Available Configurations
| Configuration | Part Number | Description |
|---|---|---|
| Direct Fit – SB | pE-400-MX-D-SB-YYY-ZZ | Direct-fit system with 365 nm DAPI excitation and a customer-specified microscope adaptor. |
| Direct Fit – MB | pE-400-MX-D-MB-YYY-ZZ | Direct-fit system with 400 nm DAPI excitation and a customer-specified microscope adaptor. |
| Light Guide – SB | pE-400-MX-L-SB-SYS-ZZ | Remote light-source configuration with 365 nm DAPI excitation for use with a 3 mm liquid light guide. |
| Light Guide – MB | pE-400-MX-L-MB-SYS-ZZ | Remote light-source configuration with 400 nm DAPI excitation for use with a 3 mm liquid light guide. |
Auckland Scientific will check the microscope, fluorescence filters and intended application to confirm the correct light-delivery method, SB or MB configuration and adaptor.
What Is Included?
- CoolLED pE-400max four-channel LED light source
- Remote manual control pod
- Power supply
- UK power cable
- Customer-specified microscope adaptor with direct-fit configurations
Liquid light guides, collimators, microscope adaptors for light-guide systems and inline excitation filter holders are selected separately.
Optional Accessories
| Accessory | Part Number | Price |
|---|---|---|
| 1.5 m long, 3 mm diameter liquid light guide | pE-1906 | Price on Request |
| 3 m long, 3 mm diameter liquid light guide | pE-1908 | Price on Request |
| pE-Universal Collimator with customer-specified microscope adaptor | pE-10400-YYY | Price on Request |
| Set of two excitation filter holders accommodating four 25 mm or 32 mm filters | pE-400-EFH-2 | Price on Request |
| USB-TTL conversion kit | pE-6501 | Price on Request |
| USB-TTL conversion kit when ordered with a pE-400max system | pE-6501S | Price on Request |
Optional Extended Warranty
| Warranty Option | Part Number | Price |
|---|---|---|
| Extension to four years of total warranty coverage | pE-400-MX-EXT-WRN-1 | Price on Request |
| Extension to five years of total warranty coverage | pE-400-MX-EXT-WRN-2 | Price on Request |
Extended warranty coverage must be ordered at the same time as the illumination system.
Technical Specifications
| LED channels | Four independently controllable channels |
|---|---|
| Spectral coverage | Approximately 365–635 nm |
| Triggering | Global and individual channel TTL; less than 10 microseconds |
| Irradiance control | 0% to 100% in 1% increments |
| Power requirement | 100–240 V AC, 50/60 Hz |
| Full white consumption | 80 W at the wall |
| Light-source dimensions | 243 × 102 × 197 mm; approximately 2.1 kg |
| Standard warranty | Three-year CoolLED manufacturer’s warranty |
Typical Applications
- High-speed multi-channel fluorescence imaging
- Live-cell and time-lapse microscopy
- Optogenetics
- Electrophysiology
- Automated fluorescence imaging
- Multi-stained sample imaging
- Quantitative fluorescence microscopy
- Manual microscope automation
- Replacement of mercury and metal-halide systems
Brochures & Technical Resources
View the CoolLED pE-400 Series brochure and supporting product information in the Auckland Scientific Technical Resource Centre.
View Brochure & Technical ResourcesExperienced Microscopy Support
Auckland Scientific brings over 21 years of microscopy sales and application experience. We provide knowledgeable support to help customers select the correct illumination system, filter configuration, light-delivery method, microscope adaptor and control integration. Support is available from specification through to installation, training and ongoing service.
Configuration & Integration Support
Please provide the microscope model, fluorescence filters, camera, imaging software and triggering requirements. Auckland Scientific will confirm the correct configuration.
Microscope, camera, fluorescence filters, liquid light guide and imaging equipment are not included unless expressly stated. Product appearance and specifications may be updated by the manufacturer without notice.