CoolLED pT-100 LED Transmitted-Light Microscope Illumination System
Advanced Transmitted-Light Illumination
CoolLED pT-100
Compact, stable and precisely controllable LED illumination for brightfield, phase contrast, darkfield, DIC and electrophysiology.
The CoolLED pT-100 is a compact transmitted-light illumination system available with broad white output or narrow-band 525 nm, 635 nm and 770 nm LEDs. It provides instant operation, stable illumination and precise irradiance control for routine microscopy and demanding research applications.
LED Illumination for Transmitted-Light Microscopy
The pT-100 brings the convenience, stability and long lifetime of LED technology to transmitted-light techniques including brightfield, darkfield, phase contrast, differential interference contrast and Dodt gradient contrast.
The broad white model is designed as an alternative to conventional 100 W halogen illumination. Its colour temperature remains consistent across the irradiance range, supporting repeatable imaging without regular colour rebalancing.
Narrow-band 525 nm, 635 nm and 770 nm versions provide application-specific benefits, including increased camera contrast, deeper penetration into thick specimens and infrared illumination for electrophysiology and specialised contrast techniques.
Key Features
- Designed for transmitted-light microscopy applications
- Choice of broad white, 525 nm, 635 nm or 770 nm illumination
- Suitable for brightfield, phase contrast, darkfield, DIC and Dodt contrast
- Instant on/off operation with no warm-up or cool-down period
- Precise irradiance control in 1% steps from 0% to 100%
- Stable and repeatable illumination for consistent imaging
- TTL control through a BNC connection on the control pod
- Triggering speed of less than 150 microseconds
- Direct-fit versions available for all four output options
- Liquid-light-guide versions available for white and 770 nm only
- Wide selection of transmitted-port microscope adaptors
- No replacement bulbs or neutral-density filters required
- Quiet, low-power and mercury-free operation
Choose the Correct Illumination
| Output | Recommended Applications |
|---|---|
| WHT – Broad White | General transmitted-light microscopy and replacement of conventional 100 W halogen illumination. Provides consistent colour temperature across the irradiance range. |
| 525 nm – Green | Higher-contrast camera imaging. The phosphor-free output is optimised around the spectral response of many scientific cameras. |
| 635 nm – Red | Brightfield imaging of thicker specimens where deeper penetration and reduced phototoxicity are beneficial. |
| 770 nm – Infrared | Infrared DIC, Dodt gradient contrast, electrophysiology and deeper imaging through thick tissue samples. |
Available Configurations
| Configuration | Part Number | Availability |
|---|---|---|
| Direct Fit | pT-100-XXX-YYY-ZZ | Available with WHT, 525 nm, 635 nm or 770 nm output. |
| 1.5 m Liquid Light Guide | pT-100-XXX-L15-ZZ | Available with WHT or 770 nm output only. Includes a fixed 1.5 m, 3 mm liquid light guide. |
| 3 m Liquid Light Guide | pT-100-XXX-L3-ZZ | Available with WHT or 770 nm output only. Includes a fixed 3 m, 3 mm liquid light guide. |
- XXX identifies WHT, 525, 635 or 770 nm output.
- YYY identifies the customer-specified microscope adaptor.
- ZZ identifies the regional power cable.
Auckland Scientific will confirm the correct output, light-delivery configuration and microscope adaptor before ordering.
Liquid-Light-Guide Delivery
White and 770 nm models can be supplied with a fixed 3 mm liquid light guide. Remote positioning is particularly useful for sensitive electrophysiology experiments because the illumination source can be positioned outside a Faraday cage, helping reduce the risk of electrical interference.
Liquid-light-guide delivery also provides compatibility with microscopes that incorporate their own collimating optics and accept a light guide directly.
What Is Included?
- CoolLED pT-100 LED light source with the selected output
- Remote manual control pod
- Power supply
- UK power cable
- Customer-specified microscope adaptor with direct-fit systems
- Fixed liquid light guide with the selected light-guide configuration
Optional Accessories
| Accessory | Part Number | Price |
|---|---|---|
| pE-Universal Collimator with customer-specified microscope adaptor | pE-10400-YYY | Price on Request |
Auckland Scientific will confirm whether a separate collimator or adaptor is required for the intended microscope.
Control Options
| Manual control | Instant on/off and irradiance adjustment in 1% steps from 0% to 100% |
|---|---|
| Remote control | Single TTL input through a BNC connection on the control pod |
| Triggering speed | Less than 150 microseconds |
Technical Specifications
| Available outputs | WHT, 525 nm, 635 nm or 770 nm |
|---|---|
| Irradiance control | 0% to 100% in 1% increments |
| Power requirement | 100–240 V AC, 50/60 Hz |
| Standby consumption | 1 W |
| Full irradiance consumption | 20 W |
| Direct-fit light source | 66 mm diameter × 128 mm long; approximately 0.42 kg |
| Control pod | 102 × 110 × 50 mm; approximately 0.55 kg |
| Standard warranty | Three-year CoolLED manufacturer’s warranty |
When viewing through microscope eyepieces, begin with the pT-100 set to its lowest irradiance and increase the output only as required for comfortable observation.
Typical Applications
- Brightfield microscopy
- Phase-contrast microscopy
- Darkfield microscopy
- Differential interference contrast
- Dodt gradient contrast
- Electrophysiology
- Infrared imaging of thick tissue samples
- Live-cell transmitted-light imaging
- Temperature-sensitive microscopy applications
- Replacement of 100 W halogen illumination
Brochures & Technical Resources
View the CoolLED pT-100 brochure and supporting product information in the Auckland Scientific Technical Resource Centre.
View Brochure & Technical ResourcesExperienced Microscopy Support
Auckland Scientific brings over 21 years of microscopy sales and application experience. We provide knowledgeable, practical support to help customers select the correct transmitted-light wavelength, light-delivery method and microscope adaptor. Support is available from initial product selection through to installation, training and ongoing service.
Configuration Support
Please provide the microscopy technique, application, microscope manufacturer and model when requesting a quotation. Auckland Scientific will help confirm the most appropriate wavelength, light-delivery configuration and adaptor.
Microscope, camera and other imaging equipment are not included unless expressly stated. Product appearance and specifications may be updated by the manufacturer without notice. Configuration must be confirmed before ordering.